Characterization of the Mel1c melatoninergic receptor in platypus (Ornithorhynchus anatinus)
Fig 3
Expression of Mel1c receptors in COS7 cells.
Subcellular localization of HA epitope-tagged Mel1c receptors. Immunofluorescence studies were performed with transfected COS7cells. COS7 cells and COS7 expressing 3HA-platypus Mel1C or 3HA-X. laevis Mel1C or 3HA-G. gallus Mel1C receptors were probed with mouse monoclonal anti-HA antibody directed against the N-terminal epitope tag present on recombinant receptor. Experiments were carried out with paraformaldehyde-fixed and non-permeabilized cells. The fluorescence images were obtained by using Alexa 488-conjugated goat anti- mouse IgG secondary antibody. COS7 cell nuclei were stained with 4′, 6′-diamidino-2-phenylindole. Native COS7 cells were used as a negative control.