Controlling cell shape on hydrogels using lift-off protein patterning
Fig 5
Lamellipodia are more exploratory for cells on LOP than on μCP patterned substrates.
Phase contrast time lapse imaging of 3 representative MDCK cells on 25 kPa PAAm gels patterned by LOP (A) and μCP (B). Cells on substrates produced by LOP follow the protein pattern border more accurately (dotted line panels A, B) and reveal more pronounced lamellipodia. (C, D) Kymograph analysis of lamellipodia kinetics along pattern edge. Single cell on LOP pattern shows increased lamellipodia protrusions and retractions within a 10 μm wide region of interest outside the protein pattern edge compared to a cell on a μCP pattern (S5 and S6 Movies). Kymographs show cells depicted in bottom row of panel A and B. Regions of interest are straightened and distorted regions at the pattern corners are cleared.