N-acetylcysteine (NAC) ameliorates Epstein-Barr virus latent membrane protein 1 induced chronic inflammation
Fig 2
IgG, IgM, IgA, IgE and complement C3c levels are increased in the transgenic tissue.
(A) The phenotypic stage was monitored in a cohort of LMP1 transgenic mice in a wild type background (LMP1CD40WT: n = 17) compared to LMP1 transgenic mice in a CD40 null background (LMP1CD40KO: n = 15) and plotted over time; error bars show SEM. (B) Ear tissue protein samples from mice of the following genotypes: L2LMP1 transgene (LMP1tg) positive (+) or negative (-), in either a CD40 null background (ko), heterozygous (het) or wild type for CD40 (wt) were western blotted. Age in days and phenotypic stage are indicated. The blots were probed with antibodies to mouse IgG, IgM, IgA, IgE and complement C3 and re-probed with antibody to GAPDH (37kD). Protein size markers in kD are indicated to the left of each panel. Note: IgG, IgE and C3c are not produced in CD40 null mice, however, IgM and IgA are induced in the LMP1 transgenic tissues.