Metabolomic profiling and stable isotope labelling of Trichomonas vaginalis and Tritrichomonas foetus reveal major differences in amino acid metabolism including the production of 2-hydroxyisocaproic acid, cystathionine and S-methylcysteine
Fig 2
Comparison of total levels of intermediates in central carbon metabolism from 13C labelled extracts.
Total metabolite levels represent the sum of the peak intensities of all isotopologues. Of metabolites detected in 13C-labelled extracts. Graphs show the log2 fold change in total peak intensities after 2 h, 6 h and 20 h growth. Results represent the mean of 3 independent cultures ± SD. Dashed line indicates the threshold for a definite difference between the species (3-fold or log2 1.6-fold change in metabolite level). Positive values represent a relative increase in T. vaginalis, negative values a relative increase. Glucose-6P, glucose-6-phosphate; Triose phosphate, dihydroxyacetone phosphate and glyceraldehyde-3-phosphate; 3-PG, 3-phoglycerate; PEP, phosphoenol pyruvate. * P ≤ 0.001 (Paired T-Test, assuming equal variance).