Rate of acyl migration in lysophosphatidylcholine (LPC) is dependent upon the nature of the acyl group. Greater stability of sn-2 docosahexaenoyl LPC compared to the more saturated LPC species
Fig 4
Stability of sn-2 acyl LPCs in organic solvent.
The sn-2 acyl LPCs were dissolved in chloroform: methanol (2:1 v/v) at a concentration of 1 mg/ml, and stored at the indicated temperature for various periods. Aliquots were taken out at the indicated time points, brought to room temperature (22°C), and subjected to LC/MS, as described in Methods. The percentage of the sn-2 acyl isomer remaining was calculated from the area counts of the two isomers. The values shown are mean ± SD of 3 separate experiments.