Rate of acyl migration in lysophosphatidylcholine (LPC) is dependent upon the nature of the acyl group. Greater stability of sn-2 docosahexaenoyl LPC compared to the more saturated LPC species
Fig 3
Stability of aqueous dispersions of sn-2 acyl LPCs at various temperatures.
The sn-2 acyl LPCs were dispersed in Tris-HCl buffer, pH 7.4 at a concentration of 2 mg/ml and incubated under nitrogen in the dark at the indicated temperature for 24 h. Aliquots were taken out at 0 h, 4 h, 8 h, 12 h, 16 h, and 24 h, brought to room temperature, diluted with equal volume of methanol and subjected to LC/MS/MS analysis as described in Methods. The percentage of the sn-2 acyl isomer remaining was calculated from the area counts of the two isomers. The values shown are mean ± SD of 3 separate experiments.