Rate of acyl migration in lysophosphatidylcholine (LPC) is dependent upon the nature of the acyl group. Greater stability of sn-2 docosahexaenoyl LPC compared to the more saturated LPC species
Fig 1
Selected LC/MS chromatograms of sn-2 acyl LPC species incubated at 37°C in Tris-HCl buffer, pH 7.4.
The aqueous solution of each sn-2 acyl LPC (2 mg/ml) was incubated at 37°C, and aliquots were taken out at the indicated time. The samples were diluted with equal volume of methanol, and analyzed by LC/MS/MS in the MRM mode, as described in Methods.