Flavin-containing monooxygenase 3 (FMO3) role in busulphan metabolic pathway
Fig 5
The effect of busulphan treatment on mRNA of FMO3 (A) and GSTA1 (B) in patients during conditioning prior to HSCT. The gene array analysis showed that FMO3 gene expression was significantly (P<0.05) increased after busulphan (Bu) conditioning (2mg/kg b.i.d. for 4 days) compared to before the start of Bu conditioning (confirmed by qRT-PCR). The expression was normalized to that of GAPDH. The inter-individual variation in the relative expression increased from 2.8-fold before Bu conditioning, to 5.6-fold by the end of the treatment. GSTA1 was also significantly up-regulated (P<0.05). Inter-individual variation in the relative expression of GSTA1 increased from 1.6-fold before Bu conditioning to 2.3-fold by the end of the treatment.