Src-family kinases negatively regulate NFAT signaling in resting human T cells
Fig 7
Calcineurin inhibitors prevent aberrant NFAT1 nuclear translocation in resting primary human T cells lacking active SFKs.
Primary human T cells obtained from healthy blood donors were treated with DMSO alone or PP2 along with FK506 or Cyclosporin A (CsA). Following subcellular fractionations, NFAT1 protein in the nuclear fraction was quantified and normalized to the nuclear actin levels. Data represent the average obtained from three different donors along with the standard deviation. *P< 0.05, **P< 0.01, Ϯ = average obtained from two donors.