Src-family kinases negatively regulate NFAT signaling in resting human T cells
Fig 5
Active Src-family kinases (SFKs) prevent aberrant NFAT1 nuclear translocation in resting primary human T cells.
(A) Immunoblot analysis of subcellular localization of NFAT1, GAPDH and Lamin in primary human T cells treated with DMSO or PP2. GAPDH and Lamin served as a loading control for cytoplasmic and nuclear fractions respectively. (B) Quantification of the NFAT1 protein in the nuclear fraction of primary human T cells obtained from four healthy blood donors either treated with DMSO or PP2. Amount of NFAT1 protein in the nuclear fraction was normalized by the Lamin protein. Each experiment was independently performed with four different donors with consistent results. *P < 0.01.