Src-family kinases negatively regulate NFAT signaling in resting human T cells
Fig 3
Src-family kinases (SFKs) regulate NFAT1 signaling in unstimulated Jurkat T cells.
(A) Immunoblot analysis of Src-kinase Lck and NFAT1 protein expression in Jurkat T cells treated with various concentrations of PP2. (B) Immunoblot analysis of subcellular localization of NFAT1, GAPDH and Lamin in Jurkat T cells treated with DMSO or PP2. GAPDH and Lamin served as a loading control for cytoplasmic and nuclear fractions respectively. (C) Quantification of NFAT1 protein in the nuclear fraction of Jurkat T cells either treated with DMSO or PP2. Amount of NFAT1 protein in the nuclear fraction was normalized by the Lamin protein level. US = unstimulated, P+I = PMA/ Ionomycin. Data represent the average of three technical replicates, and the standard deviation is shown. Each experiment was independently performed three times with similar results. *P< 0.05; **P< 0.01.