Src-family kinases negatively regulate NFAT signaling in resting human T cells
Fig 2
Inhibition of Src-family kinases (SFKs) enhances distal T cell receptor (TCR) signaling in two different human T cell lines.
Activation of distal TCR signaling was measured in two different human T cell lines by assessing (A, C) IL-2 release and (B, D) CD25 surface expression following DMSO or PP2 (5μg/ml) treatment and PMA/Ionomycin stimulation. Jurkat cells = panels A and B. HuT78 = panels C and D. Data represent the average of three technical replicates, and the standard deviation is shown. Each experiment was independently performed three times with similar results. *P< 0.01.