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Modulation of benzylisoquinoline alkaloid biosynthesis by heterologous expression of CjWRKY1 in Eschscholzia californica cells

Fig 4

Effects of overexpression of the CjWRKY1 gene on bHLH transcription factor genes in transgenic California poppy cell lines.

The expression levels of EcbHLH1-1 and EcbHLH1-2 were determined by quantitative RT-PCR. Nine biological replicates were used of each cell line. An asterisk indicates a significant difference compared with VC cell lines (*P < 0.05; two-tailed paired Student’s t-test).

Fig 4

doi: https://doi.org/10.1371/journal.pone.0186953.g004