Modulation of benzylisoquinoline alkaloid biosynthesis by heterologous expression of CjWRKY1 in Eschscholzia californica cells
Fig 2
CjWRKY1-overexpressing transgenic California poppy cells were established.
(A) Three transgenic cell lines were cultured for 5 days. (B) The medium from CjWRKY1-OX cell lines showed clear brown pigmentation. (C) Constitutive expression of CjWRKY1 in transgenic cell lines. The transcript level of CjWRKY1 was determined by quantitative RT-PCR. Nine biological replicates were used of each cell line.