Scaffold dependent histone deacetylase (HDAC) inhibitor induced re-equilibration of the subcellular localization and post-translational modification state of class I HDACs
Fig 5
Increase in cytosolic HDAC1 is irreversible up to 24 hours.
MCF-7 cells were treated with 10 μM panobinostat for 12 hours, the compound was then removed and cells allowed to recover for 24 hours. A) Western blot analysis of the abundance of HDAC1 in the cytosolic, nuclear soluble, and chromatin bound fractions. B) Densitometry analysis of the abundance of HDAC1 normalized to GAPDH (cytosolic fraction) or to TATA-binding protein (TBP, nuclear soluble and chromatin bound fractions). * Statistically significant difference compared with DMSO control (Student’s t-test, P<0.01). Western blots shown are representative of at least two independent experiments. HDAC1 fold change is presented as the mean of at least two independent experiments ± standard deviation.