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Distinguishing neuronal from astrocytic subcellular microstructures using in vivo Double Diffusion Encoded 1H MRS at 21.1 T

Fig 5

Examples of the voxel localization and of spectra obtained in representative DDE RE-MRS in vivo experiments.

(a) Coronal (upper panel) and sagittal (lower panels) views of the brain via a low-resolution T2-weighted water-based imaging experiment, applied without (left most images) and with (middle panels) a LASER localization module identical to the one that was subsequently applied in DDE RE-MRS. A clearer view of the voxel is presented in the right-hand panel of (a), where the voxel image is overlaid on a darkened image of the brain. (b) Representative spectrum arising from the 125 μL voxel shown in (a), collected in ~6.5 min and showing the targeted resonances, together with small residuals from water and from a nearby taurine resonance.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0185232.g005