The histone variant macroH2A confers functional robustness to the intestinal stem cell compartment
Fig 2
MacroH2A DKO intestine during homeostasis.
(A) Left: Representative Ki67 immunohistochemistry of macroH2A WT and DKO proximal jejunum. 10x objective. Right: Average Ki67+ crypt height in macroH2A WT vs. DKO proximal jejunum. N = 3 mice per condition, medians, quartiles and ranges of values shown. (B) Average height in microns of crypt-villus axis (distance from base of crypt to tip of villus) of macroH2A WT vs. DKO proximal jejunum (C) Average number of crypts per mm of macroH2A WT vs. DKO proximal jejunum. (D) Representative immunofluorescence and immunohistochemical images of jejunum of macroH2A WT or DKO mice stained for lysozyme (Paneth cells), chromogranin A (enteroendocrine cells), alkaline phosphatase (enterocytes), or alcian blue (goblet cells). Immunofluorescence counterstained with DAPI (blue). Lysozyme, chromogranin A and alkaline phosphatase: 20x objective, alcian blue: 10x objective. Scale bars = 100μm. ns = not significant, Student’s t-test.