In vitro and in vivo effects of 2,4 diaminoquinazoline inhibitors of the decapping scavenger enzyme DcpS: Context-specific modulation of SMN transcript levels
Fig 8
Volcano plots of differentially regulated genes detected by RNA-Seq analysis of N2a cells treated with RG3039 or its 7-methyl analog PF-06802336 with summary of number of genes significantly (P<0.05) changed.
Plates of cells (in quadruplicate) were treated with 1 μM of either compound or DMSO (1 μM) for 24 hours before isolation of polyA RNA and RNA Seq analysis. In each case, the—Log10 of the corrected P value for the difference between compound- and DMSO-treated samples for each detectable gene was plotted vs the Log2 of the fold difference. The table summarizes the numbers of significantly (P<0.05) differentially-regulated genes irrespective of the fold change and as a percentage of the total numbers of genes detectable. A total of 18,409 genes were detectable.