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Identification and molecular characterization of a metagenome-derived L-lysine decarboxylase gene from subtropical soil microorganisms

Fig 4

12% (w/v) SDS-PAGE analysis of recombinant Ldc1E.

Lane 1, molecular mass standards. Lane 2, total protein of E. coli BL21 (DE3) pLysS harboring empty pET30a(+) (control). Lane 3, total protein of E. coli BL21 (DE3) pLysS harboring the recombinant ldc1E in pET30a(+). and Lane 4, protein was purified using the Ni-NTA column method. The black arrow indicates the recombinant Ldc1E.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0185060.g004