Application of droplet digital PCR for quantitative detection of Spiroplasma citri in comparison with real time PCR
Fig 5
Diagnostic performance comparison between qPCR and ddPCR assays for citrus stubborn disease diagnosis.
ROC curve indicates better diagnostic performance of ddPCR assays compared to the qPCR assays for differentiating between healthy and S. citri infected leaf samples with significantly (P <0.05) broader AUC 0.867 and 0.967 with (a) SP1 and (b) ORF1 primers, respectively.