Strontium-doped hydroxyapatite polysaccharide materials effect on ectopic bone formation
Fig 5
mRNA expression of early and late osteoblastic gene in MSCs cultured on the three different matrices.
Runx 2 (A) and OPN (B) expression were quantified by qPCR after 1, 3 and 7 days of hMSCs cultured on Matrix-HA, Matrix-8Sr-HA and Matrix-50Sr-HA. Three separate experiments were performed and each assay was done in duplicate. Data were expressed in relative expression normalized to P0 expression, compared to day 1. Runx2 (A) and OPN (B) gene expression were set as “1.0” at Day 1 for the three scaffolds. Results were expressed as average ± SD. The symbol * denotes p< 0.05; ** denotes p<0.01 and *** indicates a significant difference with p< 0.001.