Biophysical analysis of the effect of chemical modification by 4-oxononenal on the structure, stability, and function of binding immunoglobulin protein (BiP)
Fig 5
(A) Changes in the near-UV CD signal of BiP at 266nm as a function of varying ATP concentration. (B) Changes in ATPase activity of protein before and after chemical modification. Blue bar is ATP only. (C) Changes in fluorescence anisotropy of peptide HTFPAVL at 482nm as a function of varying BiP concentration. (D) Changes in light scattering at 350 nm due to the aggregation of citrate synthase as a function of incubation time at 43°C. Blue indicates citrate synthase alone. In all panels, black and red colors indicate the data for wild-type BiP and chemically modified BiP, respectively.