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Chloroplast digestion and the development of functional kleptoplasty in juvenile Elysia timida (Risso, 1818) as compared to short-term and non-chloroplast-retaining sacoglossan slugs

Fig 5

Digestive activity as indicated by chloroplast and lysosome abundance in specimens of 4, 10, 15 and 25 Days Post-Metamorphosis (DPM) El. timida.

Black shapes indicate starved animals and grey shapes indicate starved-then-fed animals. Diamonds show chloroplast abundance inside the digestive gland tubule, here referred to as the region of interest (ROI), triangles denote lysosome abundance inside the ROI and circles depict lysosome abundance outside the ROI. All values are given in percent coverage, the percentage of tissue either inside or outside the ROI where fluorescent signal was measured as compared to the total area covered by the tissue. Overlapping shapes were illustrated directly next to each other for clarity, but were measured on the same day. A–digestive activity in 4 DPM juveniles. B– 10 DPM juveniles. C– 15 DPM juveniles. D– 25 DPM juveniles.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0182910.g005