Quantification of sympathetic hyperinnervation and denervation after myocardial infarction by three-dimensional assessment of the cardiac sympathetic network in cleared transparent murine hearts
Fig 3
Spatial relationship between sympathetic nerves and coronary vessels.
X-Y plane (A) and three-dimensional (B) images of tyrosine hydroxylase (green)-stained and α-smooth muscle actin (red)-stained heart samples. Images demonstrate that nerve fibers are distributed around coronary vessels. Images are obtained using a confocal microscope (LSM 700, Carl Zeiss). Scale bars represent 200 μm. (C) High resolution images of sympathetic nervous distribution around the coronary vessels. (D) Higher magnified view of the boxed region in C. Numerous hairline nerve branches are allocated around the coronary vessels. Images are acquired using a confocal microscope (LSM 880, Carl Zeiss) with Airyscan (Carl Zeiss). Scale bars represent 50 μm (C) and 20 μm (D).