Biochemical characterization of recombinant influenza A polymerase heterotrimer complex: Endonuclease activity and evaluation of inhibitors
Fig 1
Biochemical characterization of purified influenza polymerase proteins.
(A) Diagram showing the interactions between the PA, PB1, and PB2 subunits. (B) Coomassie staining of purified PA proteins. 1 μg of PAN (lane 1), PA (lane 2), PA/PB1 dimer (lane 3) and PA/PB1/PB2 trimer (lane 4) were analyzed by Coomassie Blue-stained SDS-PAGE. Molecular weights of protein standards are indicated on the left. (C) Western blot detection of purified proteins. 100 ng of PA, PA/PB1 dimer, and PA/PB1/PB2 trimer were subjected to SDS-PAGE and transferred to PVDF membrane. The membrane was blotted with anti-FLAG, anti-PB1 and anti-PB2 antibodies for detection of PA, PB1 and PB2 proteins respectively.