Ribbon scanning confocal for high-speed high-resolution volume imaging of brain
Fig 2
Ribbon scanning confocal microscopy is faster than conventional confocal microscopy and produces equivalent quality and reduced stitch artifacts.
A single 10x10 millimeter dry mount coronal section of rat brain was imaged on (A) the Caliber I.D. ribbon scanning confocal (Olympus 20x, 0.7NA) (B) the Nikon A1R using 4x averaging (Nikon 20x, 0.75NA) and (C) a swept field confocal using high-speed triggered acquisition (Nikon 20x, 0.75NA). Panels D-F are zoomed regions from panel A-C, respectively. The zoomed regions correspond to the red box in panel A. The times of acquisition for various microscopes and modes is recorded in panel G. Acquisition on a (H) Nikon A1 using a Galvano line scanner or (I) swept field confocal using standard sequential acquisition with line averaging led to better quality than the corresponding panels B and C but with additional time penalties.