Characterization of kinesin switch I mutations that cause hereditary spastic paraplegia
Fig 5
Mn2+-induced rescue of S203C activity.
A) ATPase rate of WT, Mg-S203C, and Mn-S203C in buffers containing Mg2+ (100% Mg2+), Mn2+ (100% Mn2+), or an equal ratio of Mg2+ to Mn2+ (50% Mg2+, 50% Mn2+). The asterisk indicates that the ATPase rate in the 1:1 ratio of divalent cation is significantly greater than the rate in Mg2+ alone (p<0.01). B) Histograms with Gaussian fits to the data of Mn-S203C in buffer conditions with the relative Mn2+:Mg2+ ratios as shown.