Morphological analysis of human umbilical vein endothelial cells co-cultured with ovarian cancer cells in 3D: An oncogenic angiogenesis assay
Fig 6
Apoptosis assay comparing co-culture of OVCAR8 with HUVECs and mono-culture of OVCAR8 at day 10.
(A) Fluorescence images of co-culture. It is noticeable that nearly no apoptotic signal was observed with thinner tubule structures, while more apoptosis was detected with the structures with larger dimensions. (B) Fluorescent images of mono-culture. Cleaved caspase 3 (magenta); DAPI (blue), HUVECs (red), and OVCAR8 (green). Scale bar: 100μm. (C) Zen localisation coefficient comparison between co-culture and mono-culture. No significant difference was observed.