Morphological analysis of human umbilical vein endothelial cells co-cultured with ovarian cancer cells in 3D: An oncogenic angiogenesis assay
Fig 4
Image processing procedure used for bright field images.
(A) The original bright field images of 3D mono-culture of OVCAR8 cells, on-top co-culture of OVCAR8 cells and HUVECs, and 3D co-culture of OVCAR8 cells and HUVECs at day 10. (B) Background corrected images of each original image. (C) Different contour lines detected in each background corrected image. (D) Segmentation of spheroids (in black colour) and tubules (in grey colour) in each image.