Optimization of a murine and human tissue model to recapitulate dermal and pulmonary features of systemic sclerosis
Fig 3
The effects of high dose BLM on dermal fibrosis.
Mice treated with BLM (60 U/kg) were sacrificed on days 7 (N = 7), 10 (N = 12), 14 (N = 7), 21 (N = 12), and 28 (N = 6). (A) Dermal thickness was measured in the dorsal skin. (B) Hydroxyproline content was measured in skin tissues shown in (A). (C) Representative H&E (left) and MT (right) images of mouse skin treated with BLM (original magnification ×40). (D) Subcutaneous adipose layer thickness was measured in the dorsal skin.