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Optimization of a murine and human tissue model to recapitulate dermal and pulmonary features of systemic sclerosis

Fig 2

The effect of low dose BLM on dermal and pulmonary fibrosis.

Mice treated with BLM (1 U/kg) were sacrificed on days 7 (N = 7), 10 (N = 4), 14 (N = 4), 21 (N = 4), and 28 (N = 4). (A) Hydroxyproline levels were measured in lungs using hydroxyproline assay. (B) Dermal thickness was measured in dorsal skin. (C) Subcutaneous adipose layer thickness was measured in dorsal skin.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0179917.g002