Optimization of a murine and human tissue model to recapitulate dermal and pulmonary features of systemic sclerosis
Fig 1
Dose-dependent changes in mouse lung and skin using the pump model.
Mice treated with the indicated doses of BLM were sacrificed on day 28. (A) The amount of collagen in the lungs was quantified using hydroxyproline assay (PBS, N = 12; 1.0 U/kg, N = 9; 10 U/kg, N = 5; 60 U/kg, N = 5; 110 U/kg, N = 5). (B) Representative images of hematoxylin and eosin (H&E) (upper) and Masson trichrome (MT) (lower) stained sections of control and BLM–treated lung tissues (original magnification ×40). (C) Dermal thickness was measured in dorsal mouse skin (PBS, N = 16; 1.0 U/kg, N = 5; 10 U/kg, N = 4; 60 U/kg, N = 5; 110 U/kg, N = 5). (D) Representative images of H&E stained sections of BLM–treated skin tissues (original magnification ×40).