Lipo-PGE1 suppresses collagen production in human dermal fibroblasts via the ERK/Ets-1 signaling pathway
Fig 4
Effects of Ets-1 on Lipo-PGE1-induced collagen inhibition in HDFs.
(A) HDFs were pretreated with or without a specific inhibitor of ERK1/2 (PD98059) for 1 h and then treated with 5 ng/mL Lipo-PGE1. Ets-1 protein was confirmed by Ets-1-specific western blot analysis. (B) HDFs were treated with Lipo-PGE1 and transfected with siRNA targeting Ets-1 or a negative control sequence. After stabilization, cells were collected and Ets-1-targeting siRNA transfection was confirmed by Ets-1—specific western blot analysis. (C) Collagen production after Ets-1-targeting siRNA transfection was detected by the Sircol assay. Data are mean ± SD of three independent experiments. *P<0.05 vs. control, §P<0.05 vs. non-treated cells transfected with negative control siRNA, #P<0.05 vs. Lipo-PGE1-treated cells transfected with negative control siRNA.