Lipo-PGE1 suppresses collagen production in human dermal fibroblasts via the ERK/Ets-1 signaling pathway
Fig 2
Lipo-PGE1 inhibited collagen gene expression and production induced by TGF- β.
(A) HDFs were pretreated with 10 ng/mL TGF-β for 2 h, before addition of 5 ng/mL Lipo-PGE1. After 24 h, total RNA was extracted and real-time RT-PCR was performed. Bars indicate mean ± SD of three independent experiments, each with triplicate samples. *P<0.05 vs. control, §P<0.05 vs. TGF-β-treated group. (B) The total collagen concentration in conditioned medium was determined by the Sircol assay after 48 h of culture. Data are mean ± SD. *P<0.05 vs. control, §P<0.05 vs. TGF-β-treated group. (C) Effects of Lipo-PGE1 on TGF-β-induced type I collagen expression. HDF cells were pretreated with TGF- β (10 ng/ml) for 2 h, and then incubated with 5 ng/mL Lipo-PGE1 for 36 h. Type I collagen production was analyzed by Western blot analysis. (D) Lipo-PGE1 inhibited TGF-β-induced phosphorylation of Smad2. HDF cells were treated with Lipo-PGE1, and then challenged with TGF- β (10 ng/ml). After 36 hours, whole cell lysates were probed with antibodies against Smad2, phospho-Smad2 in Western blots. The band intensities were quantitated and data are mean ± SD. *P<0.01 vs. control, §P<0.01 vs. TGF-β-treated group.