Effect of hyperbaric oxygen on BDNF-release and neuroprotection: Investigations with human mesenchymal stem cells and genetically modified NIH3T3 fibroblasts as putative cell therapeutics
Fig 1
Proliferation of HBO-treated cells.
The effect of hyperbaric oxygen (HBO) treatment on the proliferation of NIH3T3 fibroblasts (A), genetically modified NIH3T3/BDNF fibroblasts (B) and MSCs (C) is depicted. HBO was applied at three different pressures: 1.0 bar (red circle and line), 1.5 bar (blue square and line) and 2.0 bar (orange triangle and line). Both NIH3T3 and NIH3T3/BDNF fibroblasts show a highly significantly increased cell number compared to the control cells (normoxic and normobaric conditions (NOR), green inverted triangle and line) after five consecutive HBO treatments. By contrast, the proliferation of MSCs started to decrease after five consecutive HBO treatments with 2.0 bar until the end of ten treatments in comparison to NOR. Values are given as mean ± standard error of the mean (SEM); N = 3, n = 3. Asterisks indicate the significance of cell numbers of the different used pressures compared to the normoxic control. Statistical assessment was performed using one-way ANOVA with Bonferroni’s multiple comparison test (n.s. = not significant; *p < 0.05; **p < 0.01; ***p < 0.001).