Regulation of the PI3K/Akt pathway during decidualization of endometrial stromal cells
Fig 7
Effect of forced expression of Akt isoforms (CA-Akt) on PRL and IGFBP1 expression.
mRNA expression of either PRL (A) IGFBP1 (B) were quantified following three days treatments. HIESC cells transfected with either Akt1, Akt2 or Akt3 Tet-On vectors were subjected to decidualization using cAMP (0.5 mM) and MPA (10μM). They were then either concomitantly treated with doxycycline (1μg/mL) in order to induce the expression of the constitutive Akt isoform construct (cAMP+MPA+Doxycycline) or cells were allowed to decidualize for 24h before doxycycline was added (cAMP+MPA+Doxycycline 24H). Cells were lysed after three days and qRT-PCR analyses were performed to quantify PRL or IGBP1 expression. β-actin mRNA expression was used as control for qPCR results. (C) Akt 1, Akt2 and Akt3 expression was quantified following three days treatments. HIESC cells transfected with either Akt1, Akt2 or Akt3 Tet-On vectors were subjected to decidualization using cAMP (0.5 mM) and MPA (10μM). They were then either concomitantly treated with doxycycline (1μg/mL) in order to induce the expression of the constitutive Akt isoform construct (cAMP+MPA+Doxycycline). Cells were lysed after three days and qRT-PCR analyses were performed to quantify Akt1, Akt2 or Akt3 expression. β-actin mRNA expression was used as control for qPCR results. Data are means ± SEM three independent experiments. Different letters represent significantly different means (p<0.05).