Regulation of the PI3K/Akt pathway during decidualization of endometrial stromal cells
Fig 3
In vitro modulation of PI3K/Akt pathway with the induction of decidualization.
Induction of decidualization was induced with cAMP (0.5 mM) and MPA (10μM) for 48h, then MG132 was added and incubated for another 24h. (A) Treatment using Mg132 increased total ubiquitination, both in control conditions and decidualized cells, indicating that the Mg132 was effective at inhibiting proteasomal degradation. (B) Total Akt and pAkt levels were measured by Western blot. (C) Individual Akt isoforms levels were assessed by Western blot. β-actin was used as loading control. Blots shown are from one representative experiment. Graphics represent Western blot densitometric analysis. (D) RT-PCR was performed for each Akt isoforms to evaluate change in mRNA transcription. Data represent means ± SEM for three independent experiments. β-actin was used as an internal control. Image shown are from one representative experiment. Graphics represent densitometric analysis. All data are means ± SEM three independent experiments. Different letters represent significantly different means (p<0.05).