Ectopic overexpression of LAPTM5 results in lysosomal targeting and induces Mcl-1 down-regulation, Bak activation, and mitochondria-dependent apoptosis in human HeLa cells
Fig 5
Localization of GFP (A) and GFP-LAPTM5 (B) in HeLa cells, and immunofluorescence staining by anti-LAPTM5 of HeLa cells transfected with pCAGGS (C) or pCAGGS-LAPTM5 (D). To observe localization of GFP and GFP-LAPTM5, HeLa cells transfected with either a GFP vector or a GFP-LAPTM5 vector were fixed with 4% paraformaldehyde for 30 min. For immunostaining of ectopically expressed LAPTM5 in HeLa cells, cells transfected with pCAGGS or pCAGGS-LAPTM5 were fixed with cold methanol and stained with rabbit polyclonal anti-human LAPTM5 antibody and DAPI as described in the Materials and Methods. Images were visualized at ×200 using the LSM 700 confocal laser scanning microscope. Representative results are presented; two additional experiments yielded similar results.