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Identification of pre-fertilization reproductive barriers and the underlying cytological mechanism in crosses among three petal-types of Jasminum sambac and their relevance to phylogenetic relationships

Fig 6

Development of the pollinated embryo sacs in reciprocal crosses among single-petal (SP), double-petal (DP) and multi-petal (MP) jasmine plants and self-pollinated plants.

(a) Unfertilized embryo sac (arrow head) of SP × DP at 3 d after pollination (DAP). (b) Unfertilized embryo sac (arrow head) of SP × MP degenerated at 5 DAP. (c) Unfertilized embryo sac (arrow head) of DP × SP at 3 DAP. (d) Unfertilized embryo sac (arrow head) of DP × MP degenerated at 5 DAP. (e) Unfertilized embryo sac (arrow head) of MP × SP at 3 DAP. (f) Unfertilized embryo sac (arrow head) of MP × DP degenerated at 4 DAP. (g) A multi-cellular embryo (arrow) of SP × SP at 5 DAP. (h) A globular embryo (arrow) of DP × DP at 10 DAP. (i) A mature fruit (arrow) of DP × DP. Abbreviation: OV: ovule. Scale bars: 100 μm

Fig 6

doi: https://doi.org/10.1371/journal.pone.0176026.g006