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Analysis of transcription factors expressed at the anterior mouse limb bud

Fig 5

Generation and phenotypic analysis of transgenic mice (Prrx1-Irx3) overexpressing Irx3 in the developing mouse limb bud.

(A) Generation of transgenic mice overexpressing Irx3 in the developing limb bud. (B) Eight fold enhanced expression of Irx3 in the limb tissue of Prrx1-Irx3 than the control (Irx3-Tg/Cre -). All data were expressed as the means ± SEM, (n = 5). *P < 0.05. (C-D) Different phenotypes of Prrx1-Irx3 autopod were classified according to (C) apparent or (D) skeletal malformation (Prrx1-Irx3: Irx3-Tg and Cre-Tg; Ctrl: Irx3-Tg and Cre (-)).

Fig 5

doi: https://doi.org/10.1371/journal.pone.0175673.g005