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Analysis of transcription factors expressed at the anterior mouse limb bud

Fig 4

Reduced expression of Irx3 in Gli3-deficient limb buds.

Whole-mount in situ analysis of Gli3xt/xt embryos (E10.5–12.5). Expression of Irx3 was reduced while no significant change was seen in the expression of Asb4 and Lhx2 in Gli3-deficient limb buds. (Control: WT or Shh +/-; FL: Forelimb; HL: Hindlimb; A: Anterior; P: Posterior).

Fig 4

doi: https://doi.org/10.1371/journal.pone.0175673.g004