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Analysis of transcription factors expressed at the anterior mouse limb bud

Fig 3

Expression pattern of candidates that are anterior regulators but expanded posteriorly in Shh-KO mouse limb buds.

Whole-mount in situ analysis was performed upon limbs of Shh-KO embryos (E10.5–11.5). Disruption of Shh resulted in posteriorly expanded expression of candidates in the developing limb bud (Control: WT or Shh +/-; FL: Forelimb; HL: Hind limb; A: Anterior; P: Posterior).

Fig 3

doi: https://doi.org/10.1371/journal.pone.0175673.g003