Relationship of the CreBC two-component regulatory system and inner membrane protein CreD with swimming motility in Stenotrophomonas maltophilia
Fig 5
The transcript changes (folds) of selected flagella-related genes between KJ and KJΔBC cells by qRT-PCR.
Total mRNA was extracted from KJ and KJΔBC logarithmic-phase cultures. cDNA was prepared by RT-PCR and used as the template for qRT-PCR. The expression of target gene transcripts in qRT-PCR were normalized to the level of expression of the 16S rRNA gene by using the ΔΔCT method. Data are the means from three independent experiments. Error bars represent the standard deviations for three triplicate samples. *, p < 0.05; **, p < 0.005.