Automated assessment of bone changes in cross-sectional micro-CT studies of murine experimental osteoarthritis
Fig 7
Osteophyte quantification using epiphyseal volume.
(A) Top views of tibial epiphyses showing medial expansion caused by osteophytes in DMM-operated (arrows and shaded regions-of-interest) compared with an unaltered contralateral (12-weeks post-surgery). At 2-weeks, the osteophyte appeared incomplete, while at 12-weeks broaden the articular surface. (B) Epiphyseal volume over time for DMM and contralateral tibiae (n = 5, * denotes comparisons between measurements in the non-operated baseline with subsequent time points post-surgery while # denotes comparisons between DMM-operated vs. contralateral tibiae at the same time point, obtained by two-way ANOVA followed by multiple comparison tests using Bonferroni correction). #P<0.05, **P<0.01 and ****P<0.0001. (C) Difference between epiphyseal volume of DMM-operated and contralateral tibiae over time expressed as a percentage difference (n = 5, *P<0.05, **P<0.01 and ***P<0.001, computed by one-way ANOVA followed by multiple comparison tests using Bonferroni correction to determine statistical differences between measurements in healthy baseline and subsequent time points).