Insight into the mechanism of action of temporin-SHa, a new broad-spectrum antiparasitic and antibacterial agent
Fig 12
CD and NMR investigation of temporins.
A, CD spectra of SHa, [K3]SHa and [A2,6,9]SHa (30 μM) in DMPC:DMPG 3:1 (mol:mol) LUVs (1 mg/ml in PBS). No ordered structure was found in PBS. CD measurements are reported as the dichroic increment (Δε) per residue. The relative helix content was deduced as the percent of helix = [Δε222 x –10], where Δε222 nm is the dichroic increment at 222 nm. B, NMR chemical shift deviations (CSDs) of Hα protons of SHa and [K3]SHa in 50 mM DHPC/25 mM DMPG bicelles. C, Residual peak volume after addition of 2% 1-palmitoyl-2-stearoyl-(12-doxyl)-sn-glycero-3-phosphocholine (12-doxylPC) paramagnetic probe. For each residue, 1 to 3 cross-peaks corresponding to HN-Hα and HN-Hβ NOE correlations were integrated. The HN protons of residues 1 and 2 were not detected. The standard deviation of peak volumes integrated for each residue is indicated.