Insight into the mechanism of action of temporin-SHa, a new broad-spectrum antiparasitic and antibacterial agent
Fig 9
Kinetics of mitochondrial membrane depolarization of L. infantum promastigotes.
Parasites were incubated for 3 h at 26°C with different concentrations of peptide (3 and 6 μM, final concentrations). Mitochondrial membrane potential was monitored by flow cytometry using the fluorescence probe TMRE. A, index of variation for SHa. B, index of variation for [K3]SHa. Negative and positive controls were assayed without peptides or with 500 μM CCCP, respectively. The index of variation is expressed in arbitrary units (a.u.). The curves were obtained from a single experiment representative of three independent experiments.