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Chronic activation of PPARα with fenofibrate reduces autophagic proteins in the liver of mice independent of FGF21

Fig 5

Effects of FB on the autophagy upstream pathways in PPARα+/+ and PARα-/- mice.

Liver samples were collected after 3 weeks of FB administration for Western blotting. (A) Effects of FB on key proteins of the mTOR pathway: mTOR (mammalian target of rapamycin), S6K (P70S6 serine/threonine kinase), 4EBP1 (eukaryotic translation initiation factor 4E-biniding protein). (B) Effects of FB on key proteins the insulin signaling pathway: Akt (protein kinase B) and GSK3β (glycogen synthase kinase 3 β). * p<0.05, ** p<0.01 control (-FB); n.s. not statistically significant, n = 5–6/group.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0173676.g005