The renal phenotype of allopurinol-treated HPRT-deficient mouse
Fig 2
(a) Representative images of ethanol-fixed eosin-stained kidney sections observed by light (left panels) and polarized microscopy (right panels). Independent of the drug dosage, renal morphology is preserved and analysis by polarized microscopy is completely negative in sections from allopurinol-treated HPRT+/- and WT animals, whereas HPRT-/- kidneys have altered structure with numerous crystals filling the tubular lumens. Scale bars = 50 μm. (b) Reversed-phase HPLC analysis of solubilized crystals collected from the tubular lumina of a HPRT-/- kidney section showing a main peak (upper panel) identified as xanthine by comparison with a commercial standard (lower panel).