The brain-specific RasGEF very-KIND is required for normal dendritic growth in cerebellar granule cells and proper motor coordination
Fig 1
No apparent gross abnormality in the cerebellar lobules or layers, or in the densely-packed granule cell layer in Nissl-stained sections of v-KIND KO cerebella.
Sagittal sections of KO and WT mice cerebella (8-week-old) were analyzed by Nissl staining. (A) The lobular structure of the whole cerebellum. Scale bar, 1 mm. (B) The layer structure of the cerebellar cortex. Scale bar, 100 μm. (C) Magnified view of the granular layer indicated by the red square in (B). Cerebellar lobules II–X. ML, molecular layer; PCL, Purkinje cell layer; GL, granular layer; WM, white matter. (D) Number of Nissl-stained puncta/1,000 μm2 in the granule cell layer of WT and KO mice. 5~10 random areas per section, 2 sections per animal, and three mice for each genotype (N = 3) was statistically analyzed. Data are shown as mean ± SEM. Two sample Student’s t-test assuming equal variances showed no statistical significance (p > 0.5).