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The Effect of Tacrolimus and Mycophenolic Acid on CD14+ Monocyte Activation and Function

Fig 3

p38MAPK phosphorylation is inhibited more in T-cells than in monocytes.

Blood samples from healthy volunteers were spiked with either vehicle, 10 ng/ml tacrolimus, 50 ng/ml tacrolimus or 200 ng/ml tacrolimus. Thereafter, the phosphorylation of p38MAPK was determined in T-cells and monocytes after 15 or 30 min. of stimulation with PMA/ionomycin. After 30 min. stimulation, T-cells were significantly more inhibited than monocytes. (Data are plotted as the mean ±SEM; n = 3) *) p < 0.05; **) p < 0.01; ***) p < 0.001.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0170806.g003